WEBA method utilizing high-performance liquid chro- matography (HPLC) was developed which quantifies ergocalciferol (vitamin Dz) or cholecalciferol (vitamin D3) after separation from the previtamin and the pho- tochemical isomers of the provitamin.
WEBReference Standard for laboratory tests as prescribed in the European Pharmacopoeia only. Established for use with the monograph(s): 0072, 0575, 0574. 2.2 Analytical information related to intended use, when applicable Chromatogram(s)/spectrum : see …
WEB1 mg of cholecalciferol is equivalent to 40 000 IU of antirachitic activity (vitamin D) in rats. CHARACTERS. Appearance: white or almost white crystals. Solubility: practically insoluble in water, freely soluble in ethanol (96 per cent), soluble in trimethylpentane and in fatty oils.
WEBCholecalciferol is a form of vitamin D, which is also called vitamin D3. The USP assay method for cholecalciferol is a normal-phase HPLC method that includes the separation of cholecalcif- erol and two isomers: pre-cholecalcif- erol and trans-cholecalciferol (Figure 1).
WEBResults: the principal peak in the chromatogram obtained with the test solution is similar in retention time to the principal peak in the chromatogram obtained with reference solution (a). D. Mix about 1 g with 10 ml of water R previously warmed to 50 °C, and cool to 20 °C.
WEBCholecalciferol (Vitamin D3) may be used as a pharmaceutical reference standard for the determination of the analyte in food, feeds, serum samples and pharmaceutical formulations by various chromatography techniques.
WEBThe chromatogram obtained with reference solution (b) shows immediately at the same level an orange principal spot which gradually becomes reddish-brown and remains so for 10 min.
WEBChromatographic system (see Chromatography 621)— The liquid chromatograph is equipped with a 254-nm detector and a 4.6-mm × 25-cm column that contains packing L3. The flow rate is about 2 mL per minute. The relative retention times are about 0.4 for precholecalciferol and 1.0 for cholecalciferol.